Real-time analysis and control of product quality during bioprocessing is a highly sought-after industrial goal. In-depth knowledge and the ability to adjust bioprocess conditions in response to that knowledge can maximize protein production and quality. However, for adequate process analytical technology (PAT) to be developed, automated, aseptic sampling and analysis is required—a need addressed by the Modular Automated Sampling Technology (MAST™) platform. This paper describes the development of this innovative technology, which allows direct transfer of aseptically collected bioreactor samples to analytical devices, providing rapid, reliable data for superior bioprocess guidance.